Dai Gyakuten Saiban 2 takes place shortly after the first game, near the the end of Japan's Meiji period. Although Susato Mikotoba returned to Japan in the first game, she ends up meeting Ryūnosuke Naruhodō again. It is implied in the announcement trailer that Dai Gyakuten Saiban 2 would tie up all the loose ends and lingering questions introduced in the previous game.
Five full-length episodes are available in the game. Additionally, like Phoenix Wright: Ace Attorney: Spirit of Justice, there are two short semi-canonical "what-if" scenarios available to those who pre-ordered the game. Instead of following the "The Adventure of..." naming scheme of the first game (of which only the first case does), this game's episode naming scheme is based on the titles of the Sherlock Holmes short story compilations (The Adventures of Sherlock Holmes, The Memoirs..., The Return..., and His Last Bow).